Entericbio

Entericbio

Real time PCR diagnostic kits for clinical microbiology

EntericBio ® realtime Stool Test dramatically reduces the time needed to test for GastroEnteritis: see process

  • Simple 3-Step Test Process - designed by Medical Scientists for Medical Scientists
  • Ease of Use - no DNA extraction, no reagent preparation, no manual pipetting,...
  • Same day results - revolutionises Faecal Pathogen Testing from previous 3-day process

EntericBio ® Test Process

These tests have been designed by Medical Scientists - for Medical Scientists.

The goal was to make the test as simple as possible, 'error proofing' at every stage.

These assays work directly from the stool sample and do not require nucleic acid extraction/purification. The entire mastermix required to perform each test is lyophilised into individual reaction wells. Each reaction well contains an internal amplification control to monitor for PCR inhibition. A positive amplification control is provided with each kit.

The EntericBio realtime® Gastro panel I assay is a molecular diagnostic test for the simultaneous detection of Salmonella enterica spp., Shigella spp., Campylobacter jejuni/coli/lari and Stx1/Stx2 from human stool samples.

The EntericBio realtime® C. difficile assay is a molecular diagnostic test for the detection of the C. difficile toxin B gene (tcdB) directly from human stool samples.

The assays work directly from the stool sample and do not require nucleic acid extraction/purification.

Process Summary; a swab is lightly coated with the stool sample, the swab is re-suspended in a tube of EntericBio Stool Preparation Solution (SPS) then heated at 97⁰C for 30 min. The heat treated samples are placed on the epmotion 5070 instrument (Eppendorf AG) for fully automated transfer of the processed samples directly to the lyophilised reaction wells using the EntericBio Gastro Panel I programme. The wells are capped and transferred to the LC480 instrument (Roche) for automated amplification, detection and analysis with the EntericBio programme. A typical run of 48 samples has a turnaround time to result of less than 3 hours with typically 30 mins hands-on time.

The Stool test requires
  • NO DNA extraction,
  • NO reagent preparation
  • NO manual pipetting
  • NO enrichment
...... truly revolutionary
 

Ordering Information

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For more information please visit: http://www.entericbio.com/